Diagnostic Kit Colloidal Gold for Calprotectin Cal Rapid Test

Diagnostic Kit (Colloidal Gold)for Calprotectin

lntended Use

lt is a colloidal gold immunochromatographic assay for the semiquantitative determination of cal from human faeces,which has important accessory diagnostic value for

inflammatory bowel disease.

Advantages of this rapid test:

1. Specimens are easy to collect

2. Easy to carry

3. Cost effective

4. Rapid

PRINCIPLE

The strip has anti cal coating McAb on test region and goat anti-rabbit IgG antibody on control region, which is fastened to membrane chromatography in advance. Lable pad is coated by colloidal gold labeled anti cal McAb and colloidal gold labeled rabbit IgG antibody in advance. When testing positive sample, the cal in sample comined with colloidal gold labeled anti cal McAb, and form immune complex, as it is allowed to migrate along the test strip, the cal conjugate complex is captured by anti cal coating McAb on the membrane and form “anti cal coating McAb-cal-colloidal gold labeled anti cal McAb” complex, a colored test band appeared on test region. The color intensity is positively correlated with the cal content. A negative sample does not produce a test band due to the absence of colloidal gold conjugate cal complex. No matter cal is present in sample or not, there is a red stripe appear on reference region and quality control region, which is regarded as quality internal enterprise standards.

Test Procedure:

1.Take out the sampling stick, inserted into the faeces sample, then put the sampling stick back, screw tight and shake well, repeat the action 3 times. Or using the sampling stick picked about 50mg faeces sample, and put in a faeces sample tube containing sample dilution, and screw tightly.

2. Use disposable pipette sampling take the thinner faeces sample from the diarrhea patient, then add 3 drops (about 100uL) to the fecal sampling tube and shake well, put aside.

3.Take out the test card from the foil bag, put it on the level table and mark it.

4.Remove the cap from the sample tube and discard the first two drops diluted sample, add 4.drops (about 100uL) no bubble diluted sample verticaly and slowly into sample well of the card with provided dispette, start timing.

5.The result should be read within 10-15 minutes, and it is invalid after 15 minutes

Production of this  Calprotectin rapid test: